Regulatory

Part:BBa_K1104205:Design

Designed by: Ting-Yun Chiang   Group: iGEM13_NYMU-Taipei   (2013-09-16)

AhpCp2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers For PCR

ahpCp2_dsbG_ahpCp_FP gcttctagag caatcgcttttactggagcaggaa
ahpCp2_RP gga ctgcagcggccgctactagta gtgttgcccttacaaatgcaacaac


PCR Time Protocol

Temperature(°C) Time(sec) Number of Cycles
94 120 --
94 15 35
53 30
72 20
72 300 --

Source

E. coli MG1655 genomic sequence

References